Cadmium as an etiology of sperm dysfunction in Holstein bulls

نویسنده

  • M. Arabi Department of Biology, Faculty of Basic Sciences, University of Shahrekord, Shahrekord, Iran
چکیده مقاله:

Oxidative stress has been identified as a crucial factor leading to male infertility largely due to peroxidativedamage to the sperm cell membrane. Cadmium (Cd) is a widespread environmental pollutant and oneof the well-known reproductive toxicants. Because of its long biological half-life (10–30 years), Cdaccumulates in the biological systems. The present study was designed to assess the concentration-dependent in vitro effect of 20 to 700 μM of Cd on the membrane integrity, motility, and acrosomal status of Holstein bull spermatozoa. We recorded a significant elevation in the malondialdehyde (MDA) level, increased lipid peroxidation (LPO) rate and a drastic decrease in the spermatocrit values, especially at the 700 μM concentration of Cd, indicating deleterious effects of Cd on the intactness of the sperm membrane. There was also a negative correlation between the LPO rate and both the percentage of motile spermatozoa (r = -0.89) and sperm viability (r = -0.86). Performing the gelatin test indicated that Cd altered the integrity of acrosomal membranes and showed an abnormal acrosome reaction. In this regard, a reverse correlation was found between the LPO rate and the percentage of halos (r = -0.96). In conclusion, Cd was proved to be a potential toxicant in the category of environmental factors that induced membrane impairments, lowered motility and viability, and decreased rate of acrosome reactions leading to bull sperm dysfunction.

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cadmium as an etiology of sperm dysfunction in holstein bulls

oxidative stress has been identified as a crucial factor leading to male infertility largely due to peroxidativedamage to the sperm cell membrane. cadmium (cd) is a widespread environmental pollutant and oneof the well-known reproductive toxicants. because of its long biological half-life (10–30 years), cdaccumulates in the biological systems. the present study was designed to assess the concen...

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عنوان ژورنال

دوره 7  شماره 3

صفحات  29- 36

تاریخ انتشار 2006-09-30

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